inhibitory effect of bacillus licheniformis on g1 and g2 aflatoxins in culture media and kilka fish meal

this study investigated the inhibitory effects of bacillus licheniformis on g1 and g2 aflatoxins in culture media and kilka fish meal. two concentrations of aflatoxins (8 and 12 ppb for g1 and g2) and (2 and 4 ml from aflatoxins standard per 50g of fish meal) were used in culture media and kilka fish meal respectively. the doses of bacillus were 3 and 4% for this experiment. changes of aflatoxins and the bacterium growth were evaluated using hplc and spectrophotometer (od: 600 ?) respectively. culture media results show that g1 and g2 concentrations decreased significantly (p < 0.05) by 99.9 and 99.4%, 99.2 and 98.2% respectively (for 3% bacillus), and 100 and 100%, 100 and 99.6%, respectively (for 4% bacillus). in fish meal, concentrations of g1 and g2 decreased significantly (p < 0.05) by 75.8 and 66.1%, 77.7 and 80.1% (for 3% bacillus with 2 and 4 ml aflatoxins standard) and 95.4 and 81.7%, 98.9 and 89.3 % (for 4% bacillus with 2 and 4 ml aflatoxins standard), respectively. the results show that bacillus licheniformis is able to decrease significantly (p < 0.05) g1 and g2 aflatoxins in culture media and fish meal and is recommended to be used as a biological tool in fish meal industry.